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KMID : 0878220150270020254
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Journal of Sasang Constitutional Medicine
2015 Volume.27 No. 2 p.254 ~ p.269
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Beneficial Effect of Taeumjowi-tang on the Cisplatin-Induced Gastrointestinal Dysfunctions in Rats
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Kim Seong-Tae
Choi Ae-Ryun
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Abstract
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Objectives: This study aimed to observe the effect of Taeumjowi-tang on the cisplatin-induced gastrointestinal dysfunctions in rats.
Methods : Four groups, each of 8 rats per group, were used in this study. Saline and distilled water treated control rats were intact vehicle control group. Delayed gastrointestinal motility was induced by intraperitoneal treatment of cisplatin 2㎎/㎏, once a week for 5 weeks(Cisplatin control group). Taeumjowi-tang aqueous extracts(TJ) were orally administered in a volume of 5㎖/㎏, once a day for 14 days from 4th cisplatin treatment(TJ group). Ondansetron 1㎎/㎏ was subcutaneously treated, in a volume of 1㎖/㎏, as same as TJ(ondansetron group). We measured the body weights, intestinal charcoal transit ratio, fecal parameters, fundus MDA(malondialdehyde), GSH(glutathione) contents and SOD(superoxide dismutase), CAT(catalase) activities, TPH(tryptophanhydroxylase) and MAO(monoamine oxidase) activities, pyloric gastrin and serotonin contents with their immunoreactive cells, colonic serotonin-immunoreactive cells, the histopathology of pylorus, fundus mucosa and colon.
Results : 1) The body weight gains, the small intestinal charcoal transfer rates, the fecal parameters(numbers, weights and water contents) were increased in TJ, ondansetron group.
2) The inhibit of fundus antioxidant defense systems by cisplatin were decreased in TJ, ondansetron group.
3) The pyloric TPH activities were increased and the pyloric MAO activities were decreased in TJ group.
4) The pyloric gastric contents and the gastrin-immunoreactive cells were increased and the pyloric serotonin contents and the pyloric and colonic serotonin-immunoreactive cells were decreased in TJ group.
5) The pylorus atrophic changes and the gastric surface erosive damage regions by cisplatin were favorably inhibited by treatment of TJ group.
Conclusions: The results obtained in this study suggest that TJ favorably retarded the cisplatin related GI(gastrointestinal) dysfunctions and constipation through modulations of GI enterochromaffin cells, serotonin and gastrin-producing cells and antioxidative systems.
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KEYWORD
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Taeumjowi-tang, Cisplatin, Ondansetron, Gastrointestinal Dysfunction, Sasang Constitution.
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